Coding

Part:BBa_K3040107

Designed by: Lai, Si-Ying   Group: iGEM19_NTHU_Taiwan   (2019-10-15)


TesA

In the microbial fatty acid biosynthesis pathway, carbon source such as glucose or fatty acid will be metabolized to Acyl-ACP. Nevertheless, the accumulation of Acyl-ACP will give negative feedback to the fatty acid biosynthesis. Thus, TesA gene which encodes a thioesterase will hydrolyze these Acyl-ACP and subsequently produce a significant level of free fatty acid. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 981
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 982
    Illegal PstI site found at 996
    Illegal NotI site found at 989
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 982
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 982
    Illegal PstI site found at 996
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None